The goal of this trial is to cleave lambda DNA and produce a number of fragments and thus gain insight into the possibilities offered by restriction enzymes.
Without these enzymes, the Human Genome project would have been hopeless.
These enzymes allow DNA to be cut at very specific sites and with very high reproducibility.
Restriction enzymes are endonucleases that catalyze the cleavage of the phosphate bonds in both strands of DNA, creating very specific DNA fragments. Included in this kit are EcoRi restriction enzymes that have been "pre-treated" to work quickly and easily, yet achieve the same results as their industrial counterpart.
EcoRi give "sticky ends" cleavages at the 5' end.
After the cleavage, the DNA is separated by gel electrophoresis.
Kit includes: instructions, Lambda DNA, Dryzymes®, Reconstitution Buffer, reaction buffer for restriction enzymes, standard DNA fragments, agarose powder and FlashBlue™ staining.
Also needed: electrophoresis apparatus, power supply, automatic pipette with tips, water bath, balance, microwave or hot plate, light box (white light), laboratory glassware, pipette pumps, rulers, distilled or demineralized water, ice.
Storage: some components require storage in a freezer.
Edvotek 212 - a link to the most recent online manual (english) is included with the product in the box.
Without these enzymes, the Human Genome project would have been hopeless.
These enzymes allow DNA to be cut at very specific sites and with very high reproducibility.
Restriction enzymes are endonucleases that catalyze the cleavage of the phosphate bonds in both strands of DNA, creating very specific DNA fragments. Included in this kit are EcoRi restriction enzymes that have been "pre-treated" to work quickly and easily, yet achieve the same results as their industrial counterpart.
EcoRi give "sticky ends" cleavages at the 5' end.
After the cleavage, the DNA is separated by gel electrophoresis.
Kit includes: instructions, Lambda DNA, Dryzymes®, Reconstitution Buffer, reaction buffer for restriction enzymes, standard DNA fragments, agarose powder and FlashBlue™ staining.
Also needed: electrophoresis apparatus, power supply, automatic pipette with tips, water bath, balance, microwave or hot plate, light box (white light), laboratory glassware, pipette pumps, rulers, distilled or demineralized water, ice.
Storage: some components require storage in a freezer.
Edvotek 212 - a link to the most recent online manual (english) is included with the product in the box.
Properties
- F778212
- F778212,212