The aim of this experiment is to understand bacterial transformation by plasmid DNA.
Students will perform bacterial transformation with plasmid DNA. Two different phenotypes of bacteria will be visible after culture. Blue and non-blue colonies.
The bacteria successfully transformed with the plasmid DNA will give a blue colony. This is because the gene responsible for the Lac + phenotype lies on the plasmid and is now written off.
No IPTG used in this experiment.
Material: for 10 groups
Time: approx. 50 minutes + overnight incubation time
Also needed: micropipettes with pipette tips, two water baths, test tube racks, thermometer, incubator, ice bath with ice, marker pen, tape, pipetting pump or balloon, microwave oven, heat-resistant gloves
Note: Some kit components need to be stored frozen.
Edvotek 221 - included with the product in the box is a link to the most recent online manual (in English)
Students will perform bacterial transformation with plasmid DNA. Two different phenotypes of bacteria will be visible after culture. Blue and non-blue colonies.
The bacteria successfully transformed with the plasmid DNA will give a blue colony. This is because the gene responsible for the Lac + phenotype lies on the plasmid and is now written off.
No IPTG used in this experiment.
Material: for 10 groups
Time: approx. 50 minutes + overnight incubation time
Also needed: micropipettes with pipette tips, two water baths, test tube racks, thermometer, incubator, ice bath with ice, marker pen, tape, pipetting pump or balloon, microwave oven, heat-resistant gloves
Note: Some kit components need to be stored frozen.
Edvotek 221 - included with the product in the box is a link to the most recent online manual (in English)
Properties
- F778221