Principles of gel filtration chromatography or called size-exclusion chromatography, the separation column is a gel consisting of pore-covered granules.
The molecules of the liquid fall into the pores as it were. The pores and granules have a set diameter. Larger particles do not fit into the pores will take the faster route past the granules and arrive earlier at the bottom of the gel.
The smaller the particles, the more often they will fall into the pores, and the longer they will take to reach the bottom of the column. This method is widely used for protein purification.
The kit contains: the sample mix, chromatography columns, elution buffer, dry matter matrix, transfer pipettes and microreagent tubes.
Also required: 50 or 100 ml beakers, 25 ml beaker or test tube, clamp on tripod bar and distilled water.
The molecules of the liquid fall into the pores as it were. The pores and granules have a set diameter. Larger particles do not fit into the pores will take the faster route past the granules and arrive earlier at the bottom of the gel.
The smaller the particles, the more often they will fall into the pores, and the longer they will take to reach the bottom of the column. This method is widely used for protein purification.
The kit contains: the sample mix, chromatography columns, elution buffer, dry matter matrix, transfer pipettes and microreagent tubes.
Also required: 50 or 100 ml beakers, 25 ml beaker or test tube, clamp on tripod bar and distilled water.
Properties
- F778108